PGD CLS (Post Graduate Diploma in Clinical Laboratory Sciences)

Review
- (4 Reviews)

Description

Post Graduate Diploma In Clinical Laboratory Sciences (PGD-CLS). This course is intended to train biological science graduates in the fields of clinical chemistry, clinical pathology and clinical microbiology laboratory techniques with adequate theoretical knowledge and practical skills

Requirements

Eligibility criteria: B.Sc. and above (with any biological science subjects)

PGD CLS (Post Graduate Diploma in Clinical Laboratory Sciences)

Module-1: GMT (General Medical Technology)

Introduction to Pathology and Medical

Technology Past present and future

Methods of blood collection (theory and Practical)

Anatomy of the vein

Notes and reading from text books

Different Anticoagulants and their use Lab Glassware

Quality Control/ QA/ Accreditation Instrumentation

Module-2: Hematology

Introduction

Erythropoiesis, Development of red cell from Birth Blast to mature cell

Leucopoiesis Development of WBC from birth

Blast to mature cells and platelet maturation

Blast to mature cell fragment

Hb formation builds it up from Succinyl CoeA And then break it down to Bilirubin. Function estimation both manual and automated: Ref ranges (RR).

WBC – Function molecular level phagocytes etc. RR of each cell Estimation.

PLT: Function molecular level and estimation, RR.

Indices: MCV, MCH, MCHC and significance in anemias.

Eosinophil Ct –Stain

Automation: Principles and must learn to read histograms and scatter plots

Hematological Dysfunction like Sickle, IM (Infectious Mononucleosis), SLE, MM (Multiple myeloma)

Anemias: Acquired/Inherited.

Anemias Leukemia’s: Acute/Chronic, FAB Classification, Case histories Coagulation Theory and Practical

Clotting Factors, Extrinsic and intrinsic clotting Cascades, build up to clot break down with Action of Plasminogen, G6PD/Sickle cell , Hb electrophoresis

Module-3: Clinical Biochemistry

Chemistry of Carbohydrates in detail with Specific reference to Glucose and Diabetes, Insulin &HbA1C

Proteins with specific reference to all total Proteins Albumin/Globulin and Electrophoresis

Lipids: with ref to Cholesterol, HDL/LDL, Triglyceride, FFA, Lipoprotein

Kidney function tests with ref to Chemistry of Urea, Uric Acid (UAC), Creatinine and their significance

Liver function tests with ref to ALT, AST, and Alkaline Phosphatase, Bilirubin, GGTP

Heart function tests with ref to CPK, SGOT, LDH, Troponins, Myoglobin, NT-Pro-BNP etc

Pancreatic function tests Amylase, Lipase, Sweat electrolytes, Blood gases and Electrolytes

Electrophoresis, Chromatography

Study of Hormones

Markers for Bone, Heart, Cancer

Automation

Module-4: Cytology

Importance of making Pap smear

Staining preparation

Pap stain

H and E technique

Hematoxylin and various hemtoxylins

Orange G

ES 36

Eosin for H and E

Differentiating various cells Malignant/benign

Histotechnique - Tissue processing,Staining and fixing,Section Prep, Problems in tissue, Processing, Safety in Lab, Staining and mounting pracs in the lab.

Module-5: Urine Analysis

Physiology of Urine Formation with the anatomy of the Kidney

Physiochemical aspects of urine and Significance from volume to pH to sugar, Acetone, protein etc.

Cellular elements of urine and their Significance viz pus cells, casts, etc.

Parasitology-Life Cycle of parasites, Nematodes – full, Trematodes – full, Cestodes – full Ovas and cysts larvas, mouth, Parts/strobila in stool their structures, Blood parasites like Malaria, Leishmania etc.

Module-6: Serology

Theory and Practical: VDRL, Widal, R.A. Latex, Pregnancy test Mono and HCGB, C- Reactive proteins, Anti-streptolysin O Titers, HBsAg, ELISA, TORCH, HIV, etc..

PCR, Infective Markers

Module-7: Blood banking (Immunohemat)

Different ABO blood groups and Rh Factors, genetics of inheritance difference Of groups of ABO, the different Rh Antigens and their significance

Use of Coombs, Albumin & Enzymes, Cross matching, incomplete antibodies their detection

Hemolytic disease of the Newborn, Rhogam

Apheresis

Importance of different blood components

Module-8: Bacteriology

Gram positive cocci and bacilli of Pathological interest with Biochemical Characters and AST

Gram negative cocci and bacilli of pathological interest with Biochemical characters and AST

Different cultures are given, their processing and staining.

Sensitivity tests

Body Fluids-Semen Examination and other body fluids Physical, chemical and Microscopic details

Extra and Advance lectures- 30 lectures in English of one hour each covering, Comprehension, letter writing, and reading essays with exam

Extra and Advance lectures- Maths will have 30 one hour lectures covering Statistics, basic and advanced needed in laboratories, and a small project with exam

Module-9: Advance lectures in – External faculty and in-house

Hematology-Reading abnormal smears viz anemia, leukemia

Hematology-How to read Histograms and Scatter plots

Biochemistry And Chemistry-ABGs and Electrolytes, and Automation

Microbiology-QC in Micro and Advance Microscopy , Virology

Endocrinology -Disease based hormones

Immunology- Vitamin D/Bone markers/ Cancer markers/ ECLIA/ ELISA/ PCR techniques

QC/QA/EQAS-Statistics with accreditation procedures – NABL/CAP

This course is designed for students desiring a career as a Clinical Laboratory Scientist in the rapidly growing stand-alone and hospital-based diagnostic laboratories. This course is also suitable for those desiring a career in the IVD / medical devices industry.

Upon the completion of the course, students will be able to:

Apply knowledge and technical skills associated with medical laboratory technology.
Perform routine clinical laboratory procedures in haematology, chemistry, immunohematology, and microbiology.
Understand quality management and standards essential for laboratory accreditation
Demonstrate the technical skills, social behaviour and professional awareness essential for a laboratory technologist.
Apply problem solving techniques in identification and correction of procedural errors, instrument malfunctions, verifying the accuracy of laboratory results, analyse and interpret clinical data
Understand professional and ethical responsibility in medical lab technology practices.
Communicate effectively and with confidence.
Awareness about grooming and soft skills in the corporate world