PGD CLS (Post Graduate Diploma in Clinical Laboratory Sciences)

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PGD CLS (Post Graduate Diploma in Clinical Laboratory Sciences) Course in HORIBA India Techical Institute

Post Graduate Diploma In Clinical Laboratory Sciences (PGD-CLS). This course is intended to train biological science graduates in the fields of clinical chemistry, clinical pathology and clinical microbiology laboratory techniques with adequate theoretical knowledge and practical skills


Eligibility criteria: B.Sc. and above (with any biological science subjects)

PGD CLS (Post Graduate Diploma in Clinical Laboratory Sciences)

  • Introduction to Pathology and Medical

  • Technology Past present and future

  • Methods of blood collection (theory and Practical)

  • Anatomy of the vein

  • Notes and reading from text books

  • Different Anticoagulants and their use Lab Glassware

  • Quality Control/ QA/ Accreditation Instrumentation

  • Introduction

  • Erythropoiesis, Development of red cell from Birth Blast to mature cell

  • Leucopoiesis Development of WBC from birth

  • Blast to mature cells and platelet maturation

  • Blast to mature cell fragment

  • Hb formation builds it up from Succinyl CoeA And then break it down to Bilirubin. Function estimation both manual and automated: Ref ranges (RR).

  • WBC – Function molecular level phagocytes etc. RR of each cell Estimation.

  • PLT: Function molecular level and estimation, RR.

  • Indices: MCV, MCH, MCHC and significance in anemias.

  • Eosinophil Ct –Stain

  • Automation: Principles and must learn to read histograms and scatter plots

  • Hematological Dysfunction like Sickle, IM (Infectious Mononucleosis), SLE, MM (Multiple myeloma)

  • Anemias: Acquired/Inherited.

  • Anemias Leukemia’s: Acute/Chronic, FAB Classification, Case histories Coagulation Theory and Practical

  • Clotting Factors, Extrinsic and intrinsic clotting Cascades, build up to clot break down with Action of Plasminogen, G6PD/Sickle cell , Hb electrophoresis

  • Chemistry of Carbohydrates in detail with Specific reference to Glucose and Diabetes, Insulin &HbA1C

  • Proteins with specific reference to all total Proteins Albumin/Globulin and Electrophoresis

  • Lipids: with ref to Cholesterol, HDL/LDL, Triglyceride, FFA, Lipoprotein

  • Kidney function tests with ref to Chemistry of Urea, Uric Acid (UAC), Creatinine and their significance

  • Liver function tests with ref to ALT, AST, and Alkaline Phosphatase, Bilirubin, GGTP

  • Heart function tests with ref to CPK, SGOT, LDH, Troponins, Myoglobin, NT-Pro-BNP etc

  • Pancreatic function tests Amylase, Lipase, Sweat electrolytes, Blood gases and Electrolytes

  • Electrophoresis, Chromatography

  • Study of Hormones

  • Markers for Bone, Heart, Cancer

  • Automation

  • Importance of making Pap smear

  • Staining preparation

  • Pap stain

  • H and E technique

  • Hematoxylin and various hemtoxylins

  • Orange G

  • ES 36

  • Eosin for H and E

  • Differentiating various cells Malignant/benign

  • Histotechnique - Tissue processing,Staining and fixing,Section Prep, Problems in tissue, Processing, Safety in Lab, Staining and mounting pracs in the lab.

  • Physiology of Urine Formation with the anatomy of the Kidney

  • Physiochemical aspects of urine and Significance from volume to pH to sugar, Acetone, protein etc.

  • Cellular elements of urine and their Significance viz pus cells, casts, etc.

  • Parasitology-Life Cycle of parasites, Nematodes – full, Trematodes – full, Cestodes – full Ovas and cysts larvas, mouth, Parts/strobila in stool their structures, Blood parasites like Malaria, Leishmania etc.

  • Theory and Practical: VDRL, Widal, R.A. Latex, Pregnancy test Mono and HCGB, C- Reactive proteins, Anti-streptolysin O Titers, HBsAg, ELISA, TORCH, HIV, etc..

  • PCR, Infective Markers

  • Different ABO blood groups and Rh Factors, genetics of inheritance difference Of groups of ABO, the different Rh Antigens and their significance

  • Use of Coombs, Albumin & Enzymes, Cross matching, incomplete antibodies their detection

  • Hemolytic disease of the Newborn, Rhogam

  • Apheresis

  • Importance of different blood components

  • Gram positive cocci and bacilli of Pathological interest with Biochemical Characters and AST

  • Gram negative cocci and bacilli of pathological interest with Biochemical characters and AST

  • Different cultures are given, their processing and staining.

  • Sensitivity tests

  • Body Fluids-Semen Examination and other body fluids Physical, chemical and Microscopic details

  • Extra and Advance lectures- 30 lectures in English of one hour each covering, Comprehension, letter writing, and reading essays with exam

  • Extra and Advance lectures- Maths will have 30 one hour lectures covering Statistics, basic and advanced needed in laboratories, and a small project with exam

  • Hematology-Reading abnormal smears viz anemia, leukemia

  • Hematology-How to read Histograms and Scatter plots

  • Biochemistry And Chemistry-ABGs and Electrolytes, and Automation

  • Microbiology-QC in Micro and Advance Microscopy , Virology

  • Endocrinology -Disease based hormones

  • Immunology- Vitamin D/Bone markers/ Cancer markers/ ECLIA/ ELISA/ PCR techniques

  • QC/QA/EQAS-Statistics with accreditation procedures – NABL/CAP

  • This course is designed for students desiring a career as a Clinical Laboratory Scientist in the rapidly growing stand-alone and hospital-based diagnostic laboratories. This course is also suitable for those desiring a career in the IVD / medical devices industry. 

    Upon the completion of the course, students will be able to:

    • Apply knowledge and technical skills associated with medical laboratory technology.
    • Perform routine clinical laboratory procedures in haematology, chemistry, immunohematology, and microbiology.
    • Understand quality management and standards essential for laboratory accreditation
    • Demonstrate the technical skills, social behaviour and professional awareness essential for a laboratory technologist. 
    • Apply problem solving techniques in identification and correction of procedural errors, instrument malfunctions, verifying the accuracy of laboratory results, analyse and interpret clinical data
    • Understand professional and ethical responsibility in medical lab technology practices.
    • Communicate effectively and with confidence.
    • Awareness about grooming and soft skills in the corporate world

    Course Features

    • Course Date : To Be Announced
    • Total Duration : 15 Months
    • Lectures : Classroom + Online
    • Language of Instruction: ENGLISH
    • Certification : Nagpur University
    • Batch Size : 20
    • Seats Available Batch 1 : 20
    • Seats Available Batch 2 : 20

    What they say


    Great and much needed initiative by HORIBA India .

    Dr Anil Handoo

    Noteworthy initiative by HORIBA India . You always exceed expectations.

    Dr Anita Singh

    The training was conducted in a structured manner and helpful in getting a clear understanding of the basics of Hematology. Although I have been using a cell counter for many years, I never knew that it provided so much information through the Histograms and Scattergrams.

    Preeti Taank

    This program has really helped me enhance my knowledge around the basics of Hematology, Automation and interpretation of Histograms and Scattergrams. This will really help me in my DMLT course and my future career in the laboratory.

    Manish Yadav

    The program was conducted jointly and smoothly , by Mr. Jaldeep Bhansali and Ms. Ruzbeh Mowdawala. It offered an excellent and easy learning experience, through interactive sessions, while addressing each participant’s query , in a very cordial manner. Look forward in the future to more such training programs.

    Dr. Meenal Rajesh Patil Ex-Prof & Head, Pathology, RG Medical College & CSM Hospital, Thane .

    ““The course opens up to a new world of training . The faculty members are so passionate to deliver the knowledge to the participants in a lucid and friendly manner. The real cases from field add value to course. The interference and QC management was really awesome. The course recommended for all medical lab technologists & laboratory professionals irrespective of years of experience. thanks to Horiba.

    Prof. Dr. C N Srinivas, Vice-President - Technical Ops, Head -Transplantation Immunology - SRL Diagnostics & Chief of Laboratory Services, Fortis Hospitals, Vadapalani, Chennai

    The training programme "Hematology Laboratory Management" has been taught in interesting manner.It was very informative training and helped me a lot to improve my knowledge. I am really Thankful to the HORIBA & HITI team for these initiative concepts, webinars and training.

    A. Sri Ram, Quality Manager, Konaseema Institute of Medical Sciences & Research Foundation (KIMS & RF),AMALAPURAM,AP

    Thanks to Mr. Bhansali & Dr. Ruzbeh for conducting a valuable extensive, informative, session series spanning from pre analytics to analytical to post analytical aspects of hematology for our lab technical team with case discussion and graph reading along with practical aspect of processing.

    Neelum Tripathi, National Director, Dr. Lal PathLabs Ltd.

    It was truly a series packed with applied knowledge essentially needed on the job on day to day basis, well-paced, interactive & engaging. Sincere thanks to the team

    Pooja Gautam, Sr. Program In-charge, Dr. Lal PathLabs Ltd.

    The session was great, especially that of Mr. Bhansali .Learnt a lot of minor as well as major details, which I was always curious to know and got almost all the answers now. Really thanks a lot for giving us such an opportunity. Looking forward for many more such trainings.

    Dr Shruti Chaudhary, Chief of Lab-Panipat, Dr. Lal PathLabs Ltd.

    It was a wonderful session. Everyone here at Moga Lab learnt a lot. Thanks for organizing!

    Dr Gaurav Arora, Chief of Lab-Moga, Dr. Lal PathLabs Ltd.

    The training was very good. The contents were extremely informative and incredibly useful. The team had done a wonderful job and I appreciate their efforts.

    Dr Taranjeet Kanda, Chief of Lab-Ludhiana, Dr. Lal PathLabs Ltd.

    Thank you, the training was so good and detailed I learned a lot from it. Trainers were so good.

    Mrs. Noble George, Technical administrator Preet Vihar, Dr. Lal PathLabs Ltd.

    Good training session and it helped in improving knowledge

    Mrs.Suman Singh, Lab Administrator, Dr. Lal PathLabs Ltd, Ghaziabad

    Very good training session , so informative and it helped in improving knowledge. Thank you so much again.

    Sangeeta Sharma, Sr. Lab Tech, Dr. Lal PathLabs Ltd, Faridabad

    The trainers were very good and understanding. Very good training session it led improvement in my working.

    Mr. Teetu Pal, Lab Tech, Dr. Lal PathLabs Ltd, Preet Vihar
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